Re - replication in the Absence of Replication Licensing Mechanisms in Drosophila Melanogaster
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چکیده
Re-replication in the Absence of Replication Licensing Mechanisms in Drosophila Melanogaster by Queying Ding Department of Pharmacology and Cancer Biology Duke University Date:_______________________ Approved: ___________________________ David M. MacAlpine, Supervisor ___________________________ Beth A. Sullivan ___________________________ Daniel J. Lew ___________________________ Dennis J. Thiele ___________________________ Xiao-Fan Wang An abstract of a dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Pharmacology and Cancer Biology in the Graduate School of Duke University
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Preferential Re-Replication of Drosophila Heterochromatin in the Absence of Geminin
To ensure genomic integrity, the genome must be duplicated exactly once per cell cycle. Disruption of replication licensing mechanisms may lead to re-replication and genomic instability. Cdt1, also known as Double-parked (Dup) in Drosophila, is a key regulator of the assembly of the pre-replicative complex (pre-RC) and its activity is strictly limited to G1 by multiple mechanisms including Cul4...
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In late mitosis and G1, Mcm2-7 are assembled onto replication origins to 'license' them for initiation. At other cell cycle stages, licensing is inhibited, thus ensuring that origins fire only once per cell cycle. Three additional factors--the origin recognition complex, Cdc6 and Cdt1--are required for origin licensing. We examine here how licensing is regulated in Xenopus egg extracts. We show...
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